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Tom Holland as Peter Parker/Spider Man

Marisa Tomei as May Parker

Sean Bean as Uncle Ben

Hailee Steinfield as Mary Jane Watson

Alexander Ludwig as Eddie Brock

Matthew McConaghey as Norman Osborn/Iron Patriot

Donald Glover as Aaron Davis/The Prowler

Michael Mando as Mac Gargan/Scorpion

Jeff Goldblum as Curt Connors/Lizard

Jeffrey Wright as Dr.Otto Octavius